PARPAL


        
 

        
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All images for each paralog, for both genetic backgrounds have been visualized using the same intensity settings.

        
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Wild-type background

                  

                  

                  
Deletion background

                  

                

                

                  
Wild-type background

                  

                  

                  
Deletion background

                  

                

                

                  
Click to download supp. data

                  

                  Table S1 - Yeast strains and plasmids used in this study
                  
A) Yeast query strain list including strain IDs, paralog ORFs and gene names. B) Genotypes for yeast query strains. C) Plasmid IDs and descriptions.

                  

                  Table S2 - Protein abundance
                  
A) Description of each column. B) Yeast query strain list including strain IDs, paralog ORFs, gene names, protein abundance mean and protein abundance of each replicate.

                  

                  Table S3 - Manual inspection of paralog pairs and negative controls
                  
A) Description of each column. B) Results of manual inspection with redistribution score provided for comparison.

                  

                  Table S4 - The redistribution, relative abundance change and relocalization
                  
A) Description of each column. B) Paralog pair ORFs and gene names; GFP tagged protein ORF and gene name; redistribution score and whether it met the 4.73 threshold (true if redistribution score ≥ 4.73 and false if redistribution score < 4.73); relative abundance change log2 fold change; relative abundance change log2 fold change q-value; relative abundance change type ns if not significant, compensation if log2 fold change ≥ 0.2, q < 0.05, dependency if log2 fold change ≤ -0.2, q < 0.05; relocalization type as assessed by visual inspection with the corresponding description.

                  

                  Table S5 - Features of paralogs
                  
A) Description of each column. B) Paralog pair ORFs and gene names; protein-protein interaction (PPI) interactors shared; genetic interaction (GI) shortest path length; PPI shortest path length; Negative GI; Trigenic interaction fraction class; GI interactors colocalize with sister paralog gene1; GI interactors colocalize with sister paralog gene2; PPI interactors colocalize with sister paralog gene1; PPI interactors colocalize with sister paralog gene2.

                  

                  Data  S1 - Features of single cells
                  
A) Gene class paralogs or random pairs; strain ID; gene name; genetic background of wild-type or deletion of its paralog; replicate number; cell ID; feature 1-128.

                  

                  Data  S2 - Protein abundance per single cell
                  
A) Gene class paralogs or random pairs; strain ID; gene name; genetic background of wild-type or deletion of its paralog; replicate number; cell ID; protein abundance.

                  

                

                

                  
SGD (Saccharomyces Genome Database): 

                  

                  

                  
Trigenic interaction fraction using single and double gene deletion mutants

                  

                  

                  

                  
Protein-protein interaction change in response to paralog deletion

                  

                  

                  

                  
Regulation of protein level in response to paralog deletion